Myc Antibody (9E11): sc-47694

c-Myc Antibody (9E11) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 408-420 of human c-Myc. c-Myc mouse monoclonal antibody (9E11) recognizes an epitope within this region and detects c-Myc-tagged fusion proteins from mouse, rat, and human sources. Anti-c-Myc monoclonal antibody (9E11) demonstrates high suitability for Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry on paraffin-embedded tissues. c-Myc (9E11) antibody is available in non-conjugated form and multiple conjugated forms including agarose, HRP, PE, FITC, and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The c-Myc protein, encoded by the MYC proto-oncogene on chromosome 8q24, is a nuclear transcription factor that plays a crucial role in regulating cell proliferation, differentiation, and apoptosis. c-Myc contains essential structural motifs in the C-terminal region, such as the basic region, helix-loop-helix, and leucine zipper domains, which are vital for DNA binding and dimerization with Max protein. The c-Myc/Max heterodimer binds to specific E-box sequences (CACGTG) in target gene promoters, activating transcription and promoting cell cycle progression. Overexpression or amplification of the c-Myc gene is a common feature in various human cancers, including Burkitt′s lymphoma, neuroblastoma, and carcinomas of the lung, breast, and colon, leading to uncontrolled cell growth and tumor development. Max can form heterodimers with other basic helix-loop-helix leucine zipper proteins like Mad1 and Mxi1; these complexes can repress transcription by recruiting co-repressors such as mSin3, thereby counteracting c-Myc activity. Understanding the complex regulatory mechanisms involving c-Myc is essential for cancer research and the development of targeted therapies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myc Antibody (9E11) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-Myc Antibody (9E11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myc Antibody (9E11) Specifications

Alternate Product Name: Myc

Product Clone Name: 9E11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 408-420

Epitope Target: c-Myc

Epitope Species: human

Product Reactivity/Detection: c-Myc p67 and c-Myc tagged fusion proteins

Species Reactivity/Detection: mouse, rat, human, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for Myc Antibody (9E11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent

The preferred Myc antibody is Myc Antibody (9E10): sc-40.