Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-Mpl CRISPR/Cas9 KO Plasmid (h): sc-403035
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-Mpl CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the c-Mpl genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-Mpl Antibody (E-7): sc-377417
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-Mpl CRISPR/Cas9 KO Plasmid (h) | sc-403035 | 20 µg | $397.00 |
Overview
MPL encodes the thrombopoietin receptor c-Mpl, a type I cytokine receptor expressed predominantly in hematopoietic stem/progenitor cells and the megakaryocytic lineage where it regulates self-renewal, survival, and platelet production. Ligand engagement activates JAK2-dependent signaling with downstream STAT, PI3K/AKT, and MAPK/ERK pathways that coordinate proliferation and differentiation programs in the bone marrow niche. Dysregulated MPL signaling and altered receptor function are associated with abnormal megakaryopoiesis and myeloid disease biology, including phenotypes linked to aberrant cytokine responsiveness and stem cell homeostasis. As a lineage-restricted receptor with well-defined pathway outputs, c-Mpl serves as a tractable node for dissecting cytokine signaling, hematopoietic fate decisions, and receptor-mediated feedback control.
c-Mpl CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MPL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MPL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MPL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish c-Mpl protein expression.
This CRISPR knockout system enables efficient generation of MPL-deficient cell models for investigation of c-Mpl signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MPL exon(s) critical for c-Mpl function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MPL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by c-Mpl CRISPR/Cas9 KO Plasmid (h) and c-Mpl CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MPL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by c-Mpl HDR Plasmid (h) and c-Mpl HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MPL homology arms to support homology-directed repair at defined MPL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.