Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-IAP1 Double Nickase Plasmid (h): sc-417778-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- c-IAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- c-IAP1 Double Nickase Plasmid (h) and c-IAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BIRC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: c-IAP1 Antibody (F-4): sc-271419
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
c-IAP1 Double Nickase Plasmid (h) | sc-417778-NIC | 20 µg | $410.00 | |||
c-IAP1 Double Nickase Plasmid (h2) | sc-417778-NIC-2 | 20 µg | $410.00 |
BIRC2 encodes cellular inhibitor of apoptosis protein 1 (c-IAP1), a RING-type E3 ubiquitin ligase that coordinates ubiquitin-dependent signaling downstream of TNF receptor superfamily members. c-IAP1 regulates canonical and non-canonical NF-κB pathways through ubiquitination of RIPK1 and modulation of NIK stability, thereby shaping inflammatory signaling, cell survival, and programmed cell death decisions. It also interfaces with apoptotic and necroptotic checkpoints by influencing caspase-8 activation and death-inducing signaling complex dynamics. Dysregulated BIRC2 expression or activity has been linked to altered apoptosis resistance and inflammatory pathway remodeling in multiple malignancies and immune-related contexts, making it a common target for mechanistic studies of TNF/NF-κB signaling.
c-IAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BIRC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BIRC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BIRC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BIRC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.