Date published: 2025-9-6

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c-Fos 항체 (D-1): sc-8047

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데이터 시트
  • c-Fos 항체(D-1) 는 마우스 monoclonal IgG2bκ, 103간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산210-335 mapping at the C-terminus of c-Fos of human 의하고 기원한다
  • 안티-c-Fos 항체 (D-1)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 c-Fos 를 감지하는 데에 추천한다.
  • c-Fos 항체(D-1)는 WB(RGB), IF, IHC(P) 및 FCM용으로 Alexa Fluor® 546 또는 Alexa Fluor® 594에 결합된 형태로 제공됩니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-8047 X, 200 µg/0.1 ml)이 있습니다.
  • c-Fos (D-1): sc-8047무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 는c-Fos 항체(D-1) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은c-Fos 항체(D-1) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

빠른 링크

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c-Fos 항체(D-1)는 WB, IP, IF, IHC(P) 및 ELISA에 의해 마우스, 랫 및 인간 기원의 c-Fos를 검출하는 IgG2b κ 마우스 단일클론 c-Fos 항체이다. c-Fos 항체(D-1)는 결합 및 비결합 항-c-Fos 항체 형태로 모두 이용 가능하다. c-Fos 종양 유전자는 2개의 독립적인 쥐 골육종 바이러스 분리주 및 조류 신아세포종 바이러스에서 초기에 검출되었다. 세포 상동체인 c-Fos는 다양한 제제에 의해 신속하고 일시적으로 유도되고 여러 유전자에 대한 전사 조절자로서 기능하는 핵인산 단백질을 암호화한다. 특정 DNA 반응 요소에 결합하는 호모-이량체 및 이종이량체를 형성하는 c-Jun 단백질과 대조적으로, c-Fos 단백질은 Jun 유전자 패밀리의 구성체와 이종이량체로만 활성이다. c-Fos의 기능적 상동체는 Fra-1, Fra-2 및 Fos B 유전자를 포함한다. 또한, 선택된 ATF/CREB 패밀리 구성체는 Fos 및 Jun과 함께 류신 지퍼 이량체를 형성할 수 있다. 상이한 이량체는 AP-1 및 CRE 부위에 대해 상이한 특이성 및 친화도를 나타낸다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

c-Fos 항체 (D-1) 참고문헌:

  1. 단백질 키나아제 D2는 스위스 3T3 세포에서 봄베신에 의해 유도된 MEK/ERK/RSK 신호, c-Fos 축적 및 DNA 합성을 강화합니다.  |  Sinnett-Smith, J., et al. 2007. J Cell Physiol. 211: 781-90. PMID: 17226786
  2. 이란의 간세포암종에서 p53, cyclinD1, RB1, c-fos 및 N-ras 유전자 발현의 면역조직화학적 분석.  |  Moghaddam, SJ., et al. 2007. World J Gastroenterol. 13: 588-93. PMID: 17278226
  3. fos 유전자 계열의 추가 구성원인 fra-2의 분리 및 특성화.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
  4. AP-1 의존성 유전자 전사에서 c-fos의 직접적인 역할.  |  Herrera, R., et al. 1990. Cell Growth Differ. 1: 483-90. PMID: 2126190
  5. fos 관련 유전자의 산물인 fra-1은 전사인자 AP-1 부위에 Jun과 협력적으로 결합합니다: 전사인자 AP-1은 여러 단백질 복합체로 구성되어 있습니다.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
  6. 신세포종에서 분리된 조류 형질 전환 레트로바이러스로, 발암 유전자로 fos 유전자를 가지고 있습니다.  |  Nishizawa, M., et al. 1987. J Virol. 61: 3733-40. PMID: 2824811
  7. 인간 원시 종양 유전자 c-jun은 전사인자 AP-1의 구조적 및 기능적 특성을 가진 DNA 결합 단백질을 암호화합니다.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
  8. c-fos 원시 종양 유전자 생성물의 염색질 연관성 및 DNA 결합 특성.  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
  9. Fos 단백질 복합체는 분리된 핵의 DNA와 결합하여 DNA 셀룰로오스에 결합합니다.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
  10. 생쥐에서 골육종의 바이러스 유도.  |  Finkel, MP., et al. 1966. Science. 151: 698-701. PMID: 5218049

주문정보

제품명카탈로그 번호 단위가격수량관심품목

c-Fos 항체 (D-1)

sc-8047
200 µg/ml
$316.00

c-Fos (D-1): m-IgG Fc BP-HRP 번들

sc-528213
200 µg Ab; 10 µg BP
$354.00

c-Fos (D-1): m-IgGκ BP-HRP 번들

sc-520536
200 µg Ab, 40 µg BP
$354.00

c-Fos 항체 (D-1) X

sc-8047 X
200 µg/0.1 ml
$316.00

c-Fos 항체 (D-1) Alexa Fluor® 546

sc-8047 AF546
200 µg/ml
$357.00

c-Fos 항체 (D-1) Alexa Fluor® 594

sc-8047 AF594
200 µg/ml
$357.00

c-Fos 항체 (D-1) Alexa Fluor® 680

sc-8047 AF680
200 µg/ml
$357.00

c-Fos 항체 (D-1) Alexa Fluor® 790

sc-8047 AF790
200 µg/ml
$357.00

Whether the antibody is suitable for the detection of liver tissue western blot of male rats, or recommend the appropriate antibody

Asked by: ZiLi
Thank you for your question.sc-8047 is recommended for WB, IP, IF, IHC (P) and ELISA to detect c-Fos from mouse, rat and human sources.It can be used in rat samples for WB experiment.If you have any further questions, please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Jiajun Z
Date published: 2022-10-31

what is the best replacement for Sc-52

Asked by: ismaila
Thank you for your question. This is one of the most popular replacement antibodies for that now discontinued polyclonal antibody, sc-52. However, we offer several different options. Please contact our Technical Service Department for a recommendation based on your intended research application(s) and species reactivity.
Answered by: Technical Support
Date published: 2019-05-25

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct? 0 answers

Asked by: Emma Li
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Answered by: Technical Support
Date published: 2019-05-25

Can this antibody be used for the detection of c-fos in the macaque monkey. If not, which of your antibodies would you recommend?

Asked by: myprefrontal
Thank you for your question. We have not tested this antibody for reactivity with macaque, and are not aware of any publications citing use of the antibody in that species. We do offer an alternative antibody, c-Fos (6-2H-2F): sc-447, that has been published at least twice for detection of c-Fos in monkey. Please see PMID's: 9108029 and 11937718 for those references. A link to that product page on our website is below. https://www.scbt.com/scbt/product/c-fos-antibody-6-2h-2f
Answered by: Technical Support
Date published: 2017-11-01

Can I still buy monoclonal antibodies from this company?

Asked by: KarenLindquist
Thank you for your question! Yes, you can certainly buy monoclonal antibodies from us! Please contact us at any time and we'd be glad to help you! You can reach us by phone at 800-457-3801 or by email at scbt@scbt.com or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-05-29

Could you please advise if this antibody is able to detect Fra proteins as is the case with the discontinued sc 253.

Asked by: Conor
Thank you for your question. The equivalent replacement mouse monoclonal antibodies to sc-253 are sc-166940: c-Fos (E-8) and sc-271243: c-Fos (C-10). Both of these mouse monoclonal antibodies are raised against the same peptide antigen used to generate the polyclonal antibody, sc-253. These recommended replacement mouse monoclonal antibodies are also recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and avian origin. Links to these products are provided below for your reference. https://www.scbt.com/scbt/product/c-fos-antibody-e-8 https://www.scbt.com/scbt/product/c-fos-antibody-c-10
Answered by: Technical Support
Date published: 2017-01-19

I want to detect c-fos in my porcine samples. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-23
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Rated 3 out of 5 by from Nucleus hypocampus mark but not hypothalamusWe tested this antibody 1:1000 in cryosectioned rat brains. Strong nuclear staining is observed in the hypocampus but not in the suprachiasmatic nucleus (SCN) where there should be staining since these animals were euthanized at the begining of their resting phase. Using cFos antibodies from other companies we were able to acquire a positive staining for the SCN. This antibody will not be usefull for us since it is not comparable with IHC using other antibodies.
Date published: 2025-04-04
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
Rated 5 out of 5 by from -We examined the effects of a 3-week treatment of olanzapine (Olz) on c-Fos protein expression in stress-relevant brain sub/ regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation an animal model of depression.
Date published: 2018-12-07
Rated 5 out of 5 by from 功能很齐全也很棒我两个月前购买了这个产品,用它来做免疫荧光看的非常清楚,我想再买一盒接着做,同时我最满意的是它的性价比,很实惠也很实用,值得购买
Date published: 2018-05-21
Rated 2 out of 5 by from It does not work wellIt did not work as well as other company's ab when I tested it by 1: 500.
Date published: 2018-04-03
Rated 2 out of 5 by from No nuclear stainingLevel of expression appears comparable to other fos antibodies, but does not stain the the nucleus and non-specific binding is present at high concentrations.
Date published: 2018-03-14
Rated 1 out of 5 by from Not satisfied!I tried a free sample of this antibody on free floating mouse brain sections and it didn't work. I used a concentration of 1:50 for the primary antibody, and 1:300 for secondary antibody (anti-mouse 488) and I didn't got any signal. Really not satisfied.
Date published: 2018-02-23
Rated 5 out of 5 by from 功能很出色我上一个月染得的fos,很出色,背景很清晰,很清晰。我上一个月染得的fos,很出色,背景很清晰,很清晰。
Date published: 2018-02-05
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c-Fos 항체 (D-1) is rated 3.9 out of 5 by 20.
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