BUBR1 Double Nickase Plasmid (h): sc-403807-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BUBR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BUBR1 Double Nickase Plasmid (h) and BUBR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BUB1B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BUBR1 Antibody (8G1): sc-47744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BUBR1 Double Nickase Plasmid (h) | sc-403807-NIC | 20 µg | $410.00 | |||
BUBR1 Double Nickase Plasmid (h2) | sc-403807-NIC-2 | 20 µg | $410.00 |
BUB1B encodes BUBR1, a core component of the spindle assembly checkpoint that monitors kinetochore–microtubule attachment and restrains APC/C activity via the mitotic checkpoint complex to prevent premature anaphase onset. Through regulation of chromosome congression, sister chromatid cohesion, and anaphase progression, BUBR1 helps maintain genome stability and limits aneuploidy arising from chromosome missegregation. Dysregulation of BUB1B/BUBR1 has been associated with chromosomal instability phenotypes and has been studied in the context of developmental growth disorders and cancer-related mitotic checkpoint defects. As a result, BUBR1 is widely used as a molecular entry point for dissecting mitotic control networks and kinetochore signaling in human cells.
BUBR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BUB1B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BUB1B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BUB1B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BUB1B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.