Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BSEP Double Nickase Plasmid (h): sc-400742-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BSEP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BSEP Double Nickase Plasmid (h) and BSEP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCB11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BSEP Antibody (F-6): sc-74500
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BSEP Double Nickase Plasmid (h) | sc-400742-NIC | 20 µg | $410.00 | |||
BSEP Double Nickase Plasmid (h2) | sc-400742-NIC-2 | 20 µg | $410.00 |
ABCB11 encodes the bile salt export pump (BSEP), a canalicular ATP-binding cassette transporter that drives ATP-dependent efflux of bile acids from hepatocytes into bile. BSEP activity is central to enterohepatic circulation and hepatic bile acid homeostasis, coordinating with bile acid synthesis, conjugation, and signaling pathways such as FXR-regulated feedback control. Disruption of ABCB11 function alters bile acid composition and intracellular accumulation, perturbing membrane integrity, oxidative stress responses, and inflammatory signaling in hepatobiliary systems. Genetic variation or reduced expression of ABCB11 is associated with cholestatic phenotypes and provides a mechanistic entry point to study transporter biology and bile acid–driven liver pathology.
BSEP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCB11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCB11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCB11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCB11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.