bradykinin Double Nickase Plasmid (h): sc-402422-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- bradykinin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- bradykinin Double Nickase Plasmid (h) and bradykinin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KNG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: bradykinin Antibody (4A1): sc-293304
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
bradykinin Double Nickase Plasmid (h) | sc-402422-NIC | 20 µg | $410.00 | |||
bradykinin Double Nickase Plasmid (h2) | sc-402422-NIC-2 | 20 µg | $410.00 |
Human KNG1 encodes high molecular weight kininogen, a multifunctional plasma protein that serves as the precursor for bradykinin generation during contact system activation. Proteolytic release of bradykinin by kallikreins engages bradykinin receptors to regulate vascular tone, endothelial permeability, pain signaling, and inflammatory mediator production, linking KNG1 to coagulation–inflammation crosstalk within the kallikrein–kinin system. KNG1 activity interfaces with intrinsic coagulation and complement-related processes through interactions with factor XII, prekallikrein, and surface binding partners on endothelium. Dysregulation of bradykinin production or signaling is implicated in edema-prone inflammatory states and vascular dysfunction, making KNG1 a relevant node for mechanistic studies of plasma protease cascades.
bradykinin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KNG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KNG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KNG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KNG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.