BLOS1 Double Nickase Plasmid (h): sc-406825-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BLOS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BLOS1 Double Nickase Plasmid (h) and BLOS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BLOC1S1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BLOS1 Antibody (E-4): sc-515444
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BLOS1 Double Nickase Plasmid (h) | sc-406825-NIC | 20 µg | $410.00 | |||
BLOS1 Double Nickase Plasmid (h2) | sc-406825-NIC-2 | 20 µg | $410.00 |
BLOC1S1 encodes BLOS1, a core subunit of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) that coordinates endosomal sorting and membrane trafficking. BLOS1 contributes to cargo delivery and recycling pathways that influence lysosome-related organelle maturation and secretory vesicle dynamics, linking it to proteostasis and cellular homeostasis. Through its roles in vesicle transport and organelle biogenesis, BLOC1S1 is studied in pathways relevant to pigmentation, immune cell granule function, and neuronal connectivity. Dysregulation of BLOC-1–associated trafficking networks has been investigated in the context of neurodevelopmental and neuropsychiatric disease mechanisms, supporting its use as a functional node for cell biology studies.
BLOS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BLOC1S1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BLOC1S1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BLOC1S1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BLOC1S1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.