beta-catenin 항체 (E-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 β-catenin을 검출할것을 권장합니다.

β-catenin 항체 (E-5): sc-7963

β-catenin 항체 (E-5) is rated 4.8 out of 5 by 104.
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데이터 시트

    • 안티-beta-catenin 항체 E-5 는 마우스 monoclonal IgG1 κ β-catenin 항체, 882간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 아미노 산680-781 mapping at the C-terminus of β-catenin of human 의하고 기원한다
    • 안티-beta-catenin 항체 (E-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 β-catenin 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-beta-catenin 항체 (E-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • β-catenin (E-5): sc-7963무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-catenin Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with β-catenin Antibody (E-5) (see ordering information below).
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这款产品测的是磷酸化和非磷酸化的β-catenin的总和吗?有哪一款产品可以直接检测非磷酸化的β-catenin吗?

Asked by: 醫醫123
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the suitable antigen retrieval method for this antibody?

Asked by: DishaV
Thank you for your question. You can find our recommended protocol with antigen retrieval methods under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2021-04-28

How much is the volume of "β-catenin Antibody (E-5) sc-7963" ?

Asked by: Farideh1
Thank you for your question. All of our normal antibodies have a total volume of 1mL.
Answered by: Tech Service 1
Date published: 2021-02-02

请问,二抗的比例范围是多少?

Asked by: 湖医学子
感谢您的提问。二抗的稀释范围是和您具体的实验方法相关的,产品主页可获取说明书,说明书中有详细的针对不同实验方法的稀释比例范围。 Thank you for your inquiry. The dilution range depend on what application used. You can get more detailed dilution information on its datasheet.
Answered by: Technical Support 8
Date published: 2018-12-21

I need to detect this protein in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary for Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from works great for western blot. Strong and clear signal at the expected molecular weight
Date published: 2022-07-19
Rated 1 out of 5 by from Not good I used the primary antibody 1:50 by IF on epithelial renal cells. Unfortunately, the signal was not detectable.
Date published: 2022-02-08
Rated 5 out of 5 by from 很好用,性价比很高 我买来用来做IP实验,该抗体效价很高,性价比比其他公司高很多,可以使用很多次。WB实验用1:1000也有较好的特异性。
Date published: 2021-12-17
Rated 5 out of 5 by from 轻易就能获得漂亮的结果 无论是做IHC还是WB都能获得满意的结果,抗体物美价廉,能够很好的满足实验求,少走弯路,希望以后会有更多好的产品!
Date published: 2021-03-15
Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 4 out of 5 by from The antibody works well in immunofluorescence The antibody performs in a good way in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA
Date published: 2020-11-10
Rated 5 out of 5 by from VERY GOOD AND STABLE FOR IF We have brought this antibody twice. It works well for both IF, IP and WB.
Date published: 2020-09-11
Rated 4 out of 5 by from NIce antibody We bought it two months ago and used it for western blotting. It worked great!
Date published: 2020-01-21
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