BAF155 항체 (DXD7) WB, IP, IF와 IHC(P)으로 human, 그리고 약하지만 mouse, rat origin의BAF155을 검출할것을 권장합니다.

BAF155 항체 (DXD7): sc-32763

BAF155 항체 (DXD7) is rated 4.2 out of 5 by 10.
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데이터 시트

    • 안티-BAF155 항체 DXD7 는 마우스 monoclonal IgG1 κ BAF155 항체, 17간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against a synthetic peptide corresponding to amino acids in the BAF155 sequence of human origin
    • BAF155 항체 (DXD7)는 WB, IP, IF and IHC(P)으로 human and, to a lesser extent, mouse, rat유래의 BAF155 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-BAF155 항체 (DXD7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-32763 X, 200 µg/0.1 ml)이 있습니다.
    • BAF155 (DXD7): sc-32763무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for BAF155 Antibody (DXD7) for WB and IHC(P) applications. These reagents are now offered in bundles with BAF155 Antibody (DXD7) (see ordering information below).

When should the TransCruz reagent sc-32763 X be used instead of the standard form sc-32763?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Baf155 (DXD7) used for western blot application 2.4ug of antibody used, detects the right band at expected molecular weight, but non specific bands at 55kDa have been observed as well.
Date published: 2018-03-07
Rated 4 out of 5 by from Good for Western Blot! We got strong bands after O/N incubation with primary at the right size. We tested it in melanoma lines. There were also some unspecific bands but far from the Baf155 specific band.
Date published: 2017-10-19
Rated 3 out of 5 by from Didn't work well on Western blot Very weak signal on Western blot. Not sure if dilution used was not high enough. Started at 1:500 and got faint bands for a signal. Had to reincubate overnight, which is not a great workflow.
Date published: 2017-09-18
Rated 4 out of 5 by from Good for IP The product is successful in endogenous BAF155 immunoprecipitation
Date published: 2016-12-07
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclear Produced robust immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Published data for IF in with human embryonic Published data for IF in with human embryonic fibroblast (HE) cells -SCBT Publication Review
Date published: 2015-05-11
Rated 5 out of 5 by from Good WB publishable data gathered with mouse Good WB publishable data gathered with mouse colon mucosa and neoplastic tissues/ COS7 cells. -SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Works well for WB with Jurkat and HeLa nuclear Works well for WB with Jurkat and HeLa nuclear extracts. -SCBT QC
Date published: 2015-04-06
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