Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATP7A Double Nickase Plasmid (h): sc-402387-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP7A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATP7A Double Nickase Plasmid (h) and ATP7A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP7A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP7A Antibody (D-9): sc-376467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP7A Double Nickase Plasmid (h) | sc-402387-NIC | 20 µg | $410.00 | |||
ATP7A Double Nickase Plasmid (h2) | sc-402387-NIC-2 | 20 µg | $410.00 |
ATP7A encodes a copper-transporting P-type ATPase that localizes primarily to the trans-Golgi network and traffics to the plasma membrane to regulate intracellular copper distribution and efflux. By delivering copper to secretory pathway enzymes and maintaining copper homeostasis, ATP7A supports the maturation and activity of multiple cuproenzymes implicated in oxidative stress control, connective tissue biology, and neurodevelopment. Its function intersects with metal ion transport, vesicular trafficking, and redox-sensitive pathways that shape cellular metabolism and signaling. Dysregulation of ATP7A is linked to inherited copper transport disorders and broader phenotypes consistent with impaired cuproenzyme function.
ATP7A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP7A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP7A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP7A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP7A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.