Date published: 2025-9-8

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Aquaporin 4/AQP4 항체 (4/18): sc-32739

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데이터 시트
  • Aquaporin 4/AQP4 항체 4/18 는 마우스 monoclonal IgG3 κ Aquaporin 4/AQP4 항체, 43간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 intracellular AQP4에서 내부에 위치한 301-318 아미노산을 항원으로 사용하였습니다.
  • WB, IP, IF, IHC(P)와 FCM으로 mouse, rat와 human origin의 AQP4을 검출할것을 권장합니다.
  • IP에 권장하는 agarose로 표기된 항체; IF, IHC(P) 와 FCM에 권장하는 phycoerythrin, FITC, Alexa Fluor® 488 또는 Alexa Fluor® 647를 제공합니다 .
  • Aquaporin 4/AQP4 (4/18): sc-32739무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 Aquaporin 4/AQP4 항체(4/18)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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AQP4 항체(4/18)는 마우스, 랫트, 인간에서 유래된 AQP4 단백질을 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 면역조직화학, 유세포분석법(FCM)을 통해 검출하는 마우스 단일클론 IgG3 카파 경쇄 항체입니다. 항-AQP4 항체(4/18)는 비접합 형태와 아가로스, 피코에리틴(PE), 플루오레세인 이소티오시아네이트(FITC), 여러 가지 알렉사 플루오르® 접합체를 포함한 다양한 접합체 형태로 제공됩니다. 아쿠아포린 4로도 알려진 AQP4는 세포막을 통과하는 물의 수송에 중요한 역할을 하며, 특히 중추신경계와 골격근에서 중요한 역할을 합니다. AQP4는 주로 빠른 트위치 근육 섬유의 근막에 위치하며, AQP4는 근육 기능과 수분 공급에 필수적인 물의 빠른 이동을 촉진합니다. 이러한 위치가 삼투압 균형을 유지하고 격렬한 신체 활동 중 근육 수축을 돕습니다. AQP4를 포함한 아쿠아포린은 물에 대한 선택적 투과성을 가능하게 하는 통로를 형성하는 필수 막 단백질이며, 아쿠아포린 기능 장애는 부종과 신경 장애를 포함한 다양한 병리 생리학적 상태를 유발할 수 있습니다. 조직에 여러 아쿠아포린 이소폼이 존재한다는 것은 신장 수분 흡수 및 뇌척수액 역학 조절과 같은 다양한 생리학적 과정에서 아쿠아포린이 중요하다는 것을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Aquaporin 4/AQP4 항체 (4/18) 참고문헌:

  1. 포유류의 아쿠아포린 물 채널 계열: 유망한 신약 표적.  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. 28킬로달톤의 적혈구 일체막 단백질에 대한 cDNA 분리: 고대 채널 계열의 일원.  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
  3. 인간 수분 채널(AQP3) 유전자의 구조와 염색체 위치.  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
  4. 인간 수은 무감수성 물 채널의 cDNA 복제, 유전자 조직 및 염색체 국소화. 뚜렷한 전사 단위의 증거.  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
  5. 바소프레신 의존적 소변 농도에 대한 인간 신장 수분 채널 아쿠아포린-2의 요구 사항.  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
  6. 인간 아쿠아포린-5 유전자. 분자 특성화 및 염색체 국소화.  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
  7. 마우스 아쿠아포린 4의 복제 및 염색체 국소화: 후보 돌연변이 표현형인 운동 실조증의 배제.  |  Turtzo, LC., et al. 1997. Genomics. 41: 267-70. PMID: 9143504
  8. 포유류 골격근의 빠른 경련 섬유에서 아쿠아포린-4의 발현.  |  Frigeri, A., et al. 1998. J Clin Invest. 102: 695-703. PMID: 9710437
  9. 아쿠아포린.  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Aquaporin 4/AQP4 항체 (4/18)

sc-32739
200 µg/ml
$316.00

Aquaporin 4/AQP4 항체 (4/18) AC

sc-32739 AC
500 µg/ml, 25% agarose
$416.00

Aquaporin 4/AQP4 항체 (4/18) Alexa Fluor® 488

sc-32739 AF488
200 µg/ml
$357.00

Aquaporin 4/AQP4 항체 (4/18) Alexa Fluor® 647

sc-32739 AF647
200 µg/ml
$357.00

Aquaporin 4/AQP4 항체 (4/18) FITC

sc-32739 FITC
200 µg/ml
$330.00

Aquaporin 4/AQP4 항체 (4/18) PE

sc-32739 PE
200 µg/ml
$343.00

Hi, does this antibody require permeabilization for staining astrocytes by flow?

Asked by: JJY2
Thank you for your question. Please see permeabilzation steps in our protocol on section C : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-11-05

I am currently using your AQP4 antibody (sc-32739) in western blots and found that ECL results showed bands at ~70kDa and fluorescence showed bands at ~35 kDa. Do you know why this might be the case?

Asked by: greeny5
Thank you for your question. For assistance please contact technical service by email scbt@scbt.com or phone (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-06-07

I am trying IHC on mouse tissue sections. Which secondary antibody would you recommend? H+L or IgG3 specific? This antibody also gives a lot of bands in western blot around 40 kDa, not exactly around the expected size of the AQP4 isoforms.

Asked by: Tejal A
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

I am a PhD student at the university of Washington. We are looking for a good antibody for mouse kidney and brain sections and wondering if you could offer a free aliquot of AQP4 Antibody (sc-32739) for us to test first.

Asked by: Chenyi
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

This is from Johns hopkins University. We plan to order AQP4 Antibody (4/18): sc-32739. First we want to make sure if the antibody can arrived tomorrow if we ordered today? Thanks!

Asked by: Anonymous
Thank you for your question. Yes, we ship all our antibody reagents overnight, so if you order a vial of sc-32739 today, you will receive it tomorrow. It is currently in stock and ready to ship to you!
Answered by: Tech Service
Date published: 2019-11-11

We used it to test for IF on frozen rat brain tissue by Paraformaldehyde perfusion ,  but  there is no positive expression.

Asked by: h123
Thank you for your question. This mouse monoclonal, AQP4 (4/18): sc-32739, is suitable for that research application. We recommend using the primary antibody at a starting dilution of 1:50 (range of 1:50-1:500). And since you are using Paraformaldehyde, we recommend including a cell permeabilization treatment (e.g., Triton X-100). A link to our recommended protocol is below. This antibody has also been cited in the literature for IF on rat brain tissue. As one example, please refer to the following publication on NCBI (PMID: 22420318). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining Please contact our Technical Support Department for additional support. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-14

Why does your Western blot data show doublet bands with AQP4 (4/18): sc-32739 monoclonal antibody?

Asked by: jenni
AQP4 consists of several phosphorylation and glycosylation sites, therefore, the doublet bands that appear on our Western blot may be due to post-translational modifications of AQP4.
Answered by: Technical Support 15
Date published: 2019-01-28

Can AQP4 (4/18): sc-32739 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, AQP4 (4/18): sc-32739 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 5 out of 5 by from Ottimo anticorpoHo recentemente provato questo anticorpo in IF, su fettine di midollo spinale di topo criostatate. Diluizione 1:200 e funziona bene, il segnale è pulito e specifico.Ottimo prodotto. Funziona molto bene anche per co-immuno
Date published: 2019-09-18
Rated 1 out of 5 by from 小鼠脑片AQP4一直染不出来我按照1:50的浓度染,没有;按照1:25染还是没有。用枸橼酸钠修复了,triton通透了,但还是没有
Date published: 2018-08-11
Rated 4 out of 5 by from work well in WB at 1:200, with hippocampuswork well in WB at 1:200, with hippocampus,but degrade in storage of -20°C
Date published: 2018-05-07
Rated 1 out of 5 by from Not working on Western blotTrial antibody did not work by Western blot on primary rat astrocyte samples. Blank blots only regardless of antibody dilution, or blocking buffer.
Date published: 2018-04-10
Rated 1 out of 5 by from Didn't work in hek293TUsed AQP4 at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. Unfortunately, we weren't able to detect anything.
Date published: 2017-09-29
Rated 5 out of 5 by from 研究血脑屏障以前用过,WB挺好的,感觉SANTA的抗体染色一直都非常好,就是不知道为啥好多抗体停产了,太可惜了。
Date published: 2017-07-28
Rated 5 out of 5 by from Nice for flow cytommetryIt worked very well for flow cytommetry. You can see a nice shift after permeabilisation with Saponin.
Date published: 2017-07-06
Rated 3 out of 5 by from no expression of AQP4 in bEND3 cellsI used this antibody sample at 1/500 dilution to detect AQP4 in bEND3 cell lysate and detectd no bands between 35 and 40 kD.
Date published: 2017-06-20
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Aquaporin 4/AQP4 항체 (4/18) is rated 4.0 out of 5 by 21.
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