Annexin VI 항체 (E-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Annexin VI을 검출할것을 권장합니다.

Annexin VI 항체 (E-5): sc-271859

Annexin VI 항체 (E-5) is rated 4.9 out of 5 by 8.
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데이터 시트

    • 안티-Annexin VI 항체 E-5 는 마우스 monoclonal IgG1 κ Annexin VI 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 human origin의 Annexin VI의 내부에 위치한 5-27의 아미노산에 해당됩니다.
    • 안티-Annexin VI 항체 (E-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Annexin VI 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Annexin VI 항체 (E-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Annexin VI (E-5): sc-271859무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Annexin VI Antibody (E-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Annexin VI Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-271859 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Ottimo funzionamento!!! L’anticorpo anti-Annexin VI (sc-271859), richiesto con l’Ordine n° 535 dell’11/11/2021 e arrivato il giorno 23/11/2021, è stato testato su un lisato di cellule HeLa, ottenuto tramite omogenizzazione manuale in una soluzione di PBS-0.1% Igepal contenente un cocktail di inibitori di proteasi. Aliquote di lisato da 2 μg, 5 μg, 10 μg e 20 μg di proteine sono state caricate su un gel di poliacrilammide al 10% e sottoposte a Western Blotting. La membrana così ottenuta è stata sottoposta a blocking (in una soluzione al 5% di non-fat dried milk in TBS-0.05% TWEEN 20) e, successivamente, incubata con l’anticorpo primario sopra riportato (1:1000 vol/vol). La membrana è poi stata incubata con un anticorpo secondario anti-mouse (1:1500 vol/vol) coniugato con perossidasi di rafano. I segnali di chemiluminescenza sono stati rivelati tramite il sistema di imaging digitale Amersham Imager 680 (GE Healthcare Life Sciences). Come mostrato nell’immagine di seguito riportata, l’anticorpo produce segnali in corrispondenza del peso molecolare dell’Annessina VI in maniera concentrazione dipendente.
Date published: 2021-11-25
Rated 5 out of 5 by from This antibody is good for WB and IP I got this antibody few month before, it is good for both WB and IP
Date published: 2017-11-20
Rated 5 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 4 out of 5 by from WORKED WELL I received this antibody as a free trial sample and used for WB and Immunoprecipitation. It worked well.
Date published: 2017-09-11
Rated 5 out of 5 by from Distinct band observed via WB using product sc Distinct band observed via WB using product sc-271859 using HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin VIII in JAR cells by WB analysis. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Produced excellent immunofluorescence membrane Produced excellent immunofluorescence membrane and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
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