Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Amphiregulin Double Nickase Plasmid (h): sc-400987-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Amphiregulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Amphiregulin Double Nickase Plasmid (h) and Amphiregulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AREG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Amphiregulin Antibody (G-4): sc-74501
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amphiregulin Double Nickase Plasmid (h) | sc-400987-NIC | 20 µg | $410.00 | |||
Amphiregulin Double Nickase Plasmid (h2) | sc-400987-NIC-2 | 20 µg | $410.00 |
Human AREG encodes amphiregulin, an epidermal growth factor receptor (EGFR/ERBB) ligand produced as a membrane-anchored precursor that can be proteolytically shed to generate a soluble signaling molecule. Amphiregulin activates EGFR-driven pathways including RAS–RAF–MEK–ERK and PI3K–AKT to regulate epithelial proliferation, survival, and tissue repair, and it contributes to paracrine communication between stromal and immune compartments. AREG expression is inducible by inflammatory cues and is frequently studied in contexts of wound healing, fibrosis, and tumor-associated signaling where EGFR pathway activity influences cellular plasticity and microenvironmental remodeling.
Amphiregulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AREG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AREG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AREG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AREG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.