Ameloblastin Double Nickase Plasmid (h): sc-403086-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ameloblastin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ameloblastin Double Nickase Plasmid (h) and Ameloblastin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AMBN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ameloblastin Antibody (H-2): sc-271012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ameloblastin Double Nickase Plasmid (h) | sc-403086-NIC | 20 µg | $410.00 | |||
Ameloblastin Double Nickase Plasmid (h2) | sc-403086-NIC-2 | 20 µg | $410.00 |
AMBN encodes ameloblastin, a secreted enamel matrix protein expressed by ameloblasts that contributes to enamel formation by regulating extracellular matrix organization, mineral deposition, and adhesion at the ameloblast–matrix interface. Ameloblastin participates in developmental programs that coordinate epithelial cell polarity, differentiation, and matrix remodeling during tooth morphogenesis, and its processing products can influence cell–matrix signaling. Disruption of AMBN function is associated with enamel defects and altered ameloblast behavior, and AMBN dysregulation is used as a marker in studies of odontogenic differentiation and related pathologies. These features make AMBN a useful locus for investigating extracellular matrix biology, mineralization processes, and epithelial–mesenchymal interactions in craniofacial research.
Ameloblastin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AMBN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AMBN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AMBN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AMBN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.