Date published: 2025-9-7

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Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5): sc-374149

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  • Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 B-5 는 마우스 monoclonal IgG2a κ Aldehyde dehydrogenase 1-A1/ALDH1A1 항체, 29간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 ALDH1A1에서 N-terminus에 위치한 105-137의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 ALDH1A1 를 감지하는 데에 추천한다.
  • 항-Aldehyde dehydrogenase 1-A1/ALDH1A1 항체(B-5)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): sc-374149무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 는 Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Aldehyde dehydrogenase 1-A1/ALDH1A1 항체(B-5) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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ALDH1A1 항체(B-5)는 마우스, 쥐 및 인간 유래의 ALDH1A1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 모노클로날 ALDH1A1 항체(ALDH1 항체라고도 지정됨)입니다. ALDH1A1 항체(B-5)는 비접합 항-ALDH1A1 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-ALDH1A1 항체 형태로 제공됩니다. 알데히드 탈수소효소(ALDH)는 알코올 유래 아세트알데히드의 해독, 코르티코스테로이드, 생체 아민 및 신경전달물질의 대사, 지질 과산화 과정에서 알데히드가 산으로 산화되는 NADP+ 의존적 산화를 매개하는 역할을 합니다. 망막 탈수소효소 1(RalDH1 또는 RALDH1), 알데히드 탈수소효소 계열 1 멤버 A1, 알데히드 탈수소효소 세포질, ALDHII, ALDH-E1 또는 ALDH E1로도 지정된 ALDH1A1은 레티노산(RA)의 생합성에 관여하는 망막 탈수소효소입니다. 세포질 또는 미토콘드리아 공간에 국한될 수 있는 두 가지 주요 간 ALDH1 이소형이 있습니다. ALDH1A2(RALDH2, RALDH2-T) 유전자는 세 가지 다른 전사체를 생성하며 레티날데히드로부터 RA의 합성을 촉매합니다. ALDH1A3(ALDH6, RALDH3, ALDH1A6)는 13개의 엑손으로 구성된 37KB 유전자로 침샘, 위, 신장에서 가장 풍부한 약 3.5KB의 주전사체를 생성합니다. ALDH3A1(위형, ALDH3, ALDHIII)은 방향족 알데히드 기질을 우선적으로 산화시키는 세포질 동이합체를 형성합니다. ALDH 유전자는 산화 스트레스 반응의 일부로 상향 조절되며, 화학요법제에 대한 대사가 가속화되는 특정 종양에 풍부하게 존재하는 것으로 보입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) 참고문헌:

  1. 선택적 알데히드 탈수소효소 1A1(ALDH1A1) 억제제의 발견 및 개발.  |  Li, B., et al. 2021. Eur J Med Chem. 209: 112940. PMID: 33328099
  2. ALDH1a1 선택적 억제제로서의 새로운 디설피람 유도체.  |  Omran, Z. 2022. Molecules. 27: PMID: 35056791
  3. 암에서의 ALDH1A1: 양방향 기능, 약물 내성 및 조절 메커니즘.  |  Yue, H., et al. 2022. Front Oncol. 12: 918778. PMID: 35814382
  4. ALDH1A1 발현은 조기 발병 대장암에서 풍부하다.  |  Venugopal, A., et al. 2022. Gastroenterology. 163: 1679-1681.e1. PMID: 35985512
  5. ALDH1A1은 레티노산 수용체 매개 DNA 중합효소 θ 발현을 강화함으로써 PARP 억제제 내성을 촉진합니다.  |  Lavudi, K., et al. 2023. NPJ Precis Oncol. 7: 66. PMID: 37429899
  6. 라이신-260의 2-하이드록시이소부티릴화 변형은 방광암 진행을 조절하기 위해 ALDH1A1 발현을 불안정하게 만듭니다.  |  Zhang, Z., et al. 2023. iScience. 26: 108142. PMID: 37867947
  7. 자폐증 환자 유래 SHANK2BY29X 돌연변이는 ALDH1A1 음성 도파민 뉴런의 발달에 영향을 미칩니다.  |  Lai, W., et al. 2024. Mol Psychiatry. 29: 3180-3194. PMID: 38704506
  8. ALDH1A1은 ZBTB7B-글리콜리시스 경로를 통해 종양 세포의 면역 회피를 촉진합니다.  |  Wang, M., et al. 2024. Cell Death Dis. 15: 568. PMID: 39107297
  9. 교모세포종 증식 및 침입에 있어서 ALDH1A1의 역할.  |  Huang, YK., et al. 2024. Chem Biol Interact. 402: 111202. PMID: 39128802
  10. 철분 독성을 통해 KRAS 표적 치료의 효과를 높이기 위해 ALDH1A1을 표적으로 삼는 방법.  |  Bian, Y., et al. 2024. Redox Biol. 77: 103361. PMID: 39317105

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5)

sc-374149
200 µg/ml
$316.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgG Fc BP-HRP 번들

sc-529674
200 µg Ab; 10 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgGκ BP-HRP 번들

sc-522639
200 µg Ab, 40 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5): m-IgG2a BP-HRP 번들

sc-547358
200 µg Ab; 10 µg BP
$354.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) AC

sc-374149 AC
500 µg/ml, 25% agarose
$416.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) HRP

sc-374149 HRP
200 µg/ml
$316.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) FITC

sc-374149 FITC
200 µg/ml
$330.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) PE

sc-374149 PE
200 µg/ml
$343.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 488

sc-374149 AF488
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 546

sc-374149 AF546
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 594

sc-374149 AF594
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 647

sc-374149 AF647
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 680

sc-374149 AF680
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) Alexa Fluor® 790

sc-374149 AF790
200 µg/ml
$357.00

Aldehyde dehydrogenase 1-A1/ALDH1A1 (B-5) 중화펩타이드

sc-374149 P
100 µg/0.5 ml
$68.00

Can this antibody be used for IF in 4% PFA fixed rat brain tissue

Asked by: bob27
Thank you for your question. sc-374149ALDH1A1 (B-5) can be used for IF in 4% PFA fixed mouse, rat or human samples.
Answered by: BlakeJ
Date published: 2025-05-14

Is this product (sc-374149) conjugated with any fluorescein like FITC or PE?

Asked by: Lina_xu
Thank you for your question. Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (B-5): sc-374149 is unconjugated. For a FITC- or PE- conjugated version of this antibody, please indicate the product code 'sc-374149 FITC' or 'sc-374149 PE', respectively, when you place an order.
Answered by: Tech Support Europe
Date published: 2022-02-21

I would like to use Alexa fluor 488-labeled anti-ALDH1 product (SC-374149 AF488) for flow cytometry. Can you let me know the corresponding isotype antibody product of this antibody?

Asked by: govltpfkj
Thank you for your question. Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (B-5) is a mouse monoclonal IgG2a, so the appropriate isotype control for the AF488 conjugate would be normal mouse IgG2a Alexa Fluor® 488: sc-3891.
Answered by: Tech Support Europe
Date published: 2021-11-02

Can this product (FITC conjugated) be used for flow cytometry?

Asked by: govltpfk
Thank you for your question. Our FITC conjugated antibodies can be used for Immunofluorescence Cell Staining and Flow Cytometry.
Answered by: Tech Support Europe
Date published: 2021-10-14

I'm getting strong band just below 75.Could you pls tell me what its likely to be. Sometimes I see multiple bands as well, its hard for me to make conclusion. FYI - these are protein lysates of rhabdomyosarcoma. Whats its exact molecular wt

Asked by: Alamelu
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

can this product be used to check WB,IHC in mouse?

Asked by: z wh
Thanks for your question. Yes, this antibody is mouse, rat and human reactive.
Answered by: Technical Support
Date published: 2018-12-14

In human liver tissue extracts ALDH1A1 shows two bands! Can you tell me what is the explanation for that? Thank you!

Asked by: 05Anca
Dear Anca, thank you very much for your question. The MW is 56 kDa. For more details about your bands which kDa and your protocol, please contact our European Office by email europe@scbt.com or phone: toll-free 00800 4573 8000.
Answered by: Technical Support Europe
Date published: 2017-09-26

What application is the blocking peptide sc-374149 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ottimo funzionamentoOttimi risultati in WB su linee di CRC: HCT15 e HCT116
Date published: 2024-12-09
Rated 4 out of 5 by from Promising IHC staining resultI got a promising IHC staining result with DAB in mouse liver
Date published: 2019-11-22
Rated 5 out of 5 by from Very goodI used it in IF, on hiPSCs-derived dopaminergic neurons differentiated in vitro: fixation with 4% PFA for 15' at RT; permeabilization 0,3% triton for 15'; blocking with 3% BSA, 2% Donkey serum, 0,1% tween for 1' and 1/2. I used a dilution 1:100 and the signal is high and without background
Date published: 2019-03-18
Rated 5 out of 5 by from ALDH1A1 antibody B-5Excelent antibody for western blot. Detected ALDH1A1 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti ALDH1A1 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 3 out of 5 by from Medium features in western blot assay.Blocking with milk for an hour. Primary antibody 1/500 PBST over night at 4ºC + anti-mouse for 1 hour. Performed western blot in 3 different breast cancer cell lines. Visible background, undefined band.
Date published: 2017-09-16
Rated 5 out of 5 by from Good for WBStrong and single band in WB at 1:500, UM-UC-3,T24 and 5637 cells
Date published: 2017-09-11
Rated 4 out of 5 by from Good result in total lysate human cell linesWe observed a marked band at approx. 60kDa and also a lower band which we haven't determined what it is. Blocking in milk for an hour; 1/1000 ALDH1A1 in PBS-T 1% at 4ºC ON; secondary atb 1/4000.
Date published: 2017-06-30
Rated 5 out of 5 by from The antibody quality is very goodMethods The cellular localization of ALDH1A1 Antibody in mouse kidney tissue was detected by immunoblot and immunohistochemistry. We found that Western Blotting can usually be diluted with 1:500.
Date published: 2017-02-09
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Aldehyde dehydrogenase 1-A1/ALDH1A1 항체 (B-5) is rated 4.7 out of 5 by 12.
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