AGTRAP Antibody (F-6): sc-271367

AGTRAP Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects AGTRAP of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AGTRAP antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AGTRAP, also known as angiotensin II receptor-associated protein, is a crucial transmembrane protein primarily located in the Golgi apparatus, endoplasmic reticulum, endocytotic vesicles, and perinuclear vesicular structures. This strategic localization is vital for AGTRAP′s role as a negative regulator of the Angiotensin II type I receptor (AT1), as AGTRAP facilitates the internalization and desensitization of the receptor, thereby modulating Angiotensin II signaling. Such regulation is essential for controlling cell proliferation and transcriptional activity stimulated by Angiotensin II, which can have significant implications in cardiovascular and renal health. AGTRAP consists of 159 amino acids and interacts with RACK1 (receptor for activated C kinase 1), a relationship that assists in recruiting AGTRAP to the AT1 receptor. Additionally, AGTRAP has two isoforms resulting from alternative splicing, which may contribute to functional diversity in various tissues, including the heart, kidney, pancreas, and thyroid.

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AGTRAP Antibody (F-6) References:

1. Cloning and characterization of ATRAP, a novel protein that interacts with the angiotensin II type 1 receptor.  |  Daviet, L., et al. 1999. J Biol Chem. 274: 17058-62. PMID: 10358057
2. ATRAP, novel AT1 receptor associated protein, enhances internalization of AT1 receptor and inhibits vascular smooth muscle cell growth.  |  Cui, T., et al. 2000. Biochem Biophys Res Commun. 279: 938-41. PMID: 11162453
3. Identification and characterization of AGTRAP, a human homolog of murine Angiotensin II Receptor-Associated Protein (Agtrap).  |  Wang, W., et al. 2002. Int J Biochem Cell Biol. 34: 93-102. PMID: 11733189
4. The angiotensin II type I receptor-associated protein, ATRAP, is a transmembrane protein and a modulator of angiotensin II signaling.  |  Lopez-Ilasaca, M., et al. 2003. Mol Biol Cell. 14: 5038-50. PMID: 12960423
5. Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane.  |  Guo, DF., et al. 2003. Biochem Biophys Res Commun. 310: 1254-65. PMID: 14559250
6. A novel angiotensin II type 1 receptor-associated protein induces cellular hypertrophy in rat vascular smooth muscle and renal proximal tubular cells.  |  Guo, DF., et al. 2004. J Biol Chem. 279: 21109-20. PMID: 14985364
7. Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation.  |  Guo, S., et al. 2005. J Biol Chem. 280: 12536-41. PMID: 15668245
8. The novel angiotensin II type 1 receptor (AT1R)-associated protein ATRAP downregulates AT1R and ameliorates cardiomyocyte hypertrophy.  |  Tanaka, Y., et al. 2005. FEBS Lett. 579: 1579-86. PMID: 15757644
9. Properdin plays a protective role in polymicrobial septic peritonitis.  |  Stover, CM., et al. 2008. J Immunol. 180: 3313-8. PMID: 18292556
10. Properdin Regulation of Complement Activation Affects Colitis in Interleukin 10 Gene-Deficient Mice.  |  Jain, U., et al. 2015. Inflamm Bowel Dis. 21: 1519-28. PMID: 25939041
11. Complement in ANCA-associated glomerulonephritis.  |  Hilhorst, M., et al. 2017. Nephrol Dial Transplant. 32: 1302-1313. PMID: 26275893
12. Properdin deficiency protects from 5-fluorouracil-induced small intestinal mucositis in a complement activation-independent, interleukin-10-dependent mechanism.  |  Jain, U., et al. 2017. Clin Exp Immunol. 188: 36-44. PMID: 28052346

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AGTRAP Antibody (F-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AGTRAP Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AGTRAP Antibody (F-6) Specifications

Alternate Product Name: AGTRAP

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-159

Epitope Target: AGTRAP

Epitope Species: human

Product Reactivity/Detection: AGTRAP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AGTRAP Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin