ACAT-1 Antibody (AT15E5): sc-517387

ACAT-1 Antibody (AT15E5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACAT-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ACAT-1 antibody (AT15E5) is available as a non-conjugated format. ACAT-1 protein, also known as acetyl-Coenzyme A acetyltransferase 1, is a crucial mitochondrial enzyme that plays a significant role in ketone body metabolism and isoleucine processing. ACAT-1 protein is located within mitochondria, where ACAT-1 facilitates acetyl-CoA conversion into acetoacetyl-CoA, a key step in ketone body synthesis and degradation. ACAT-1′s mitochondrial localization is important for regulating energy metabolism, particularly during fasting or low carbohydrate intake, when ketone bodies serve as an alternative energy source for brain and heart tissues. Deficiencies in ACAT-1 can lead to serious metabolic disorders, including β-ketothiolase deficiency, characterized by ketoacidotic attacks. Additionally, ACAT-2, a cytosolic counterpart, is vital for cholesterol synthesis, highlighting these enzymes′ interconnected roles in lipid metabolism.

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ACAT-1 Antibody (AT15E5) References:

1. Identification, purification and characterization of an acetoacetyl-CoA thiolase from rat liver peroxisomes.  |  Antonenkov, VD., et al. 2000. Eur J Biochem. 267: 2981-90. PMID: 10806397
2. Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine.  |  Chang, CC., et al. 2000. J Biol Chem. 275: 28083-92. PMID: 10846185
3. Phylogenetic analysis of the thiolase family. Implications for the evolutionary origin of peroxisomes.  |  Igual, JC., et al. 1992. J Mol Evol. 35: 147-55. PMID: 1354266
4. High resolution crystal structures of human cytosolic thiolase (CT): a comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I.  |  Kursula, P., et al. 2005. J Mol Biol. 347: 189-201. PMID: 15733928
5. Phylogenetic analysis of eukaryotic thiolases suggests multiple proteobacterial origins.  |  Peretó, J., et al. 2005. J Mol Evol. 61: 65-74. PMID: 15980957
6. Human urotensin II accelerates foam cell formation in human monocyte-derived macrophages.  |  Watanabe, T., et al. 2005. Hypertension. 46: 738-44. PMID: 16172428
7. Inborn errors of isoleucine degradation: a review.  |  Korman, SH. 2006. Mol Genet Metab. 89: 289-99. PMID: 16950638
8. Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.  |  Haapalainen, AM., et al. 2007. Biochemistry. 46: 4305-21. PMID: 17371050
9. Expression of two isozymes of acyl-coenzyme A: cholesterol acyltransferase-1 and -2 in clear cell type renal cell carcinoma.  |  Matsumoto, K., et al. 2008. Int J Urol. 15: 166-70. PMID: 18269457
10. Rat liver peroxisomes catalyze the initial step in cholesterol synthesis. The condensation of acetyl-CoA units into acetoacetyl-CoA.  |  Thompson, SL. and Krisans, SK. 1990. J Biol Chem. 265: 5731-5. PMID: 1969406
11. ACAT-1-Regulated Cholesteryl Ester Accumulation Modulates Gemcitabine Resistance in Biliary Tract Cancer.  |  Ueno, G., et al. 2022. Ann Surg Oncol. 29: 2899-2909. PMID: 34994902
12. Assignment of the human cytosolic acetoacetyl-coenzyme A thiolase (ACAT2) gene to chromosome 6q25.3-q26.  |  Masuno, M., et al. 1996. Genomics. 36: 217-8. PMID: 8812443

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACAT-1 Antibody (AT15E5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ACAT-1 Antibody (AT15E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACAT-1 Antibody (AT15E5) Specifications

Alternate Product Name: ACAT-1

Product Clone Name: AT15E5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 34-427

Epitope Target: ACAT-1

Epitope Species: human

Product Reactivity/Detection: ACAT-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ACAT-1 Antibody (AT15E5): N/A