AATK CRISPR/Cas9 KO Plasmid (m): sc-418928
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AATK CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AATK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AATK Antibody (L-37): sc-100436
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AATK CRISPR/Cas9 KO Plasmid (m) | sc-418928 | 20 µg | $397.00 |
Overview
Aatk encodes apoptosis-associated tyrosine kinase (AATK), a serine/threonine kinase enriched in the nervous system that supports neuronal differentiation, neurite extension, and survival decisions during development and stress. AATK activity interfaces with apoptosis and growth-factor signaling programs, influencing cytoskeletal remodeling and transcriptional responses that shape cell fate. Dysregulated AATK expression or signaling has been linked to altered neuronal maturation and vulnerability to degeneration, making it relevant to studies of neurodevelopmental mechanisms and neurodegenerative disease biology. In mouse systems, Aatk serves as a tractable node for dissecting kinase-dependent control of neuronal morphology and stress-induced cell death pathways.
AATK CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aatk gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aatk together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aatk open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AATK protein expression.
This CRISPR knockout system enables efficient generation of Aatk-deficient cell models for investigation of AATK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aatk exon(s) critical for AATK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aatk genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AATK CRISPR/Cas9 KO Plasmid (m) and AATK CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aatk locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AATK HDR Plasmid (m) and AATK HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aatk homology arms to support homology-directed repair at defined Aatk target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.