A1Up Double Nickase Plasmid (h): sc-407973-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- A1Up Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- A1Up Double Nickase Plasmid (h) and A1Up Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBQLN4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: A1Up Antibody (A333): sc-136145
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A1Up Double Nickase Plasmid (h) | sc-407973-NIC | 20 µg | $410.00 | |||
A1Up Double Nickase Plasmid (h2) | sc-407973-NIC-2 | 20 µg | $410.00 |
UBQLN4 (A1Up) encodes a ubiquitin-like adaptor protein implicated in proteostasis by coupling polyubiquitinated substrates to degradation and quality-control pathways. A1Up is associated with ubiquitin-dependent turnover and interacts functionally with components of the ubiquitin–proteasome system and autophagy-linked clearance, influencing protein homeostasis under basal and stress conditions. Through these roles, UBQLN4 can impact cellular responses to proteotoxic stress, protein aggregation, and remodeling of signaling networks that depend on regulated protein stability. Dysregulation of ubiquitin-mediated protein quality control has broad relevance to neurodegeneration, cancer biology, and other conditions where impaired proteostasis contributes to pathology, making UBQLN4 a useful target for mechanistic studies.
A1Up Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBQLN4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBQLN4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBQLN4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBQLN4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.