3β-HSD6 CRISPR/Cas9 KO Plasmid (m): sc-420970
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 3β-HSD6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the 3β-HSD6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
3β-HSD6 CRISPR/Cas9 KO Plasmid (m) | sc-420970 | 20 µg | $397.00 |
Overview
Mouse Hsd3b6 encodes 3β-HSD6, a hydroxysteroid dehydrogenase that catalyzes key redox steps in steroid hormone biosynthesis by interconverting 3β-hydroxysteroids and 3-ketosteroids. This activity supports steroidogenic flux and contributes to maintaining cellular steroid homeostasis, impacting downstream nuclear receptor signaling and broader endocrine-regulated transcriptional programs. Altered 3β-HSD family function can shift androgen, estrogen, and corticosteroid precursor pools, linking this enzymatic node to studies of reproductive biology, adrenal/gonadal physiology, and metabolic regulation. Hsd3b6 is therefore relevant for dissecting how steroidogenic enzymes shape tissue-specific signaling networks and phenotypes in mouse model systems.
3β-HSD6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hsd3b6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hsd3b6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hsd3b6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish 3β-HSD6 protein expression.
This CRISPR knockout system enables efficient generation of Hsd3b6-deficient cell models for investigation of 3β-HSD6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hsd3b6 exon(s) critical for 3β-HSD6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hsd3b6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by 3β-HSD6 CRISPR/Cas9 KO Plasmid (m) and 3β-HSD6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hsd3b6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by 3β-HSD6 HDR Plasmid (m) and 3β-HSD6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hsd3b6 homology arms to support homology-directed repair at defined Hsd3b6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.