Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α E-catenin Double Nickase Plasmid (h): sc-401151-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α E-catenin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α E-catenin Double Nickase Plasmid (h) and α E-catenin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTNNA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α E-catenin Antibody (G-11): sc-9988
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α E-catenin Double Nickase Plasmid (h) | sc-401151-NIC | 20 µg | $410.00 | |||
α E-catenin Double Nickase Plasmid (h2) | sc-401151-NIC-2 | 20 µg | $410.00 |
CTNNA1 encodes human α E-catenin, a core component of adherens junctions that links cadherin–β-catenin complexes to the actin cytoskeleton to stabilize cell–cell adhesion and regulate cortical tension. By coordinating junctional remodeling with actomyosin dynamics, α E-catenin contributes to epithelial polarity, tissue morphogenesis, and contact-dependent control of cell behavior. CTNNA1 function intersects with pathways governing cytoskeletal organization and junctional signaling, including crosstalk with Wnt/β-catenin regulation at the membrane and in transcriptional outputs. Altered CTNNA1 activity or expression has been associated with defects in adhesion and invasion-relevant phenotypes in cancer biology, as well as epithelial integrity changes relevant to developmental and hematologic contexts.
α E-catenin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTNNA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTNNA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTNNA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTNNA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.