ZO-1 Antibody (R40.76): sc-33725
- ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a ZO-1 antibody, cited in 220 publications, provided at 200 µg/ml
- raised against DOC-insoluble junctional ribbons isolated from rat liver
- ZO-1 Antibody (R40.76) is recommended for detection of ZO-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-ZO-1 Antibody (R40.76) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZO-1 Antibody (R40.76). This work is in progress.
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ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a antibody designed to detect the Zonula Occludens-1 protein, also known as Tight Junction Protein 1 (TJP1), in mouse, rat, and human samples using western blotting (WB), immunoprecipitation (IP), and PAR assays. Anti-ZO-1 antibody (R40.76) is raised against DOC-insoluble junctional ribbons isolated from rat liver, ensuring reliable recognition of the ZO-1 epitope. ZO-1 is a critical component of tight junctions, which are essential for maintaining the integrity and selective permeability of epithelial and endothelial cell layers. As a member of the membrane-associated guanylate kinase (MAGUK) family, ZO-1 interacts with other tight junction proteins such as occludin, claudins, ZO-2, and ZO-3 through its PDZ, SH3, and guanylate kinase domains, facilitating both structural stability and signal transduction. These interactions link the tight junction complexes to the actin cytoskeleton, playing a vital role in regulating cell polarity, barrier function, and cellular communication. Dysregulation of ZO-1 has been associated with various pathological conditions, including cancer metastasis, where ZO-1 expression is notably reduced in many breast cancer cell lines, as well as in cardiovascular and neurological disorders. Anti-ZO-1 antibody (R40.76) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatile options for different experimental applications. ZO-1 monoclonal antibody (R40.76) enables researchers to investigate the expression, localization, and functional dynamics of ZO-1, thereby advancing our understanding of cellular junctions and their role in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZO-1 Antibody (R40.76) References:
- Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin. | Itoh, M., et al. 1999. J Biol Chem. 274: 5981-6. PMID: 10026224
- Manner of interaction of heterogeneous claudin species within and between tight junction strands. | Furuse, M., et al. 1999. J Cell Biol. 147: 891-903. PMID: 10562289
- Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3. | Wittchen, ES., et al. 1999. J Biol Chem. 274: 35179-85. PMID: 10575001
- Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. | Itoh, M., et al. 1999. J Cell Biol. 147: 1351-63. PMID: 10601346
- Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. | Furuse, M., et al. 1994. J Cell Biol. 127: 1617-26. PMID: 7798316
- Cell signalling: MAGUK magic. | Anderson, JM. 1996. Curr Biol. 6: 382-4. PMID: 8723338
- ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. | Haskins, J., et al. 1998. J Cell Biol. 141: 199-208. PMID: 9531559
- Loss of the tight junction MAGUK ZO-1 in breast cancer: relationship to glandular differentiation and loss of heterozygosity. | Hoover, KB., et al. 1998. Am J Pathol. 153: 1767-73. PMID: 9846967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZO-1 Antibody (R40.76) | sc-33725 | 200 µg/ml | $322.00 | |||
ZO-1 Antibody (R40.76) AC | sc-33725 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZO-1 Antibody (R40.76) HRP | sc-33725 HRP | 200 µg/ml | $322.00 | |||
ZO-1 Antibody (R40.76) FITC | sc-33725 FITC | 200 µg/ml | $336.00 | |||
ZO-1 Antibody (R40.76) PE | sc-33725 PE | 200 µg/ml | $349.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 488 | sc-33725 AF488 | 200 µg/ml | $364.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 546 | sc-33725 AF546 | 200 µg/ml | $364.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 594 | sc-33725 AF594 | 200 µg/ml | $364.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 647 | sc-33725 AF647 | 200 µg/ml | $364.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 680 | sc-33725 AF680 | 200 µg/ml | $364.00 | |||
ZO-1 Antibody (R40.76) Alexa Fluor® 790 | sc-33725 AF790 | 200 µg/ml | $364.00 |