ZC3H4 Double Nickase Plasmid (m): sc-436020-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZC3H4 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZC3H4 Double Nickase Plasmid (m) and ZC3H4 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zc3h4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZC3H4 Double Nickase Plasmid (m) | sc-436020-NIC | 20 µg | $410.00 |
Zc3h4 encodes the zinc finger CCCH-type containing protein ZC3H4, an RNA-associated factor implicated in nuclear gene regulation and RNA metabolic processes. ZC3H4 has been linked to control of transcriptional output through interactions with RNA processing machinery, contributing to proper mRNA maturation and turnover and influencing gene expression programs. By shaping RNA fate and transcription-associated processes, ZC3H4 can affect cell state transitions, stress responses, and proliferative signaling networks. Dysregulation of RNA processing and transcriptional control is broadly relevant to disease biology, making Zc3h4 a useful locus for mechanistic studies of gene expression control in mouse models.
ZC3H4 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zc3h4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zc3h4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zc3h4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zc3h4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.