Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Vimentin Double Nickase Plasmid (m): sc-423676-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vimentin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Vimentin Double Nickase Plasmid (m) and Vimentin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Vim. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vimentin Antibody (E-5): sc-373717
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vimentin Double Nickase Plasmid (m) | sc-423676-NIC | 20 µg | $410.00 | |||
Vimentin Double Nickase Plasmid (m2) | sc-423676-NIC-2 | 20 µg | $410.00 |
Vim encodes vimentin, a type III intermediate filament protein that organizes the cytoskeletal network and helps maintain cell shape, mechanical integrity, and organelle positioning in mesenchymal and motile cell states. Vimentin dynamically remodels during cell division, migration, and adhesion, interfacing with actin and microtubules and integrating signaling pathways involved in epithelial–mesenchymal transition, wound repair, and inflammatory responses. In mouse models, altered vimentin expression or filament organization is linked to changes in immune cell trafficking, fibrosis-associated remodeling, and metastasis-related phenotypes, making Vim a useful node for studying cytoskeletal–signaling crosstalk. Its broad expression in stromal and immune compartments also supports investigations into tissue architecture and microenvironmental regulation.
Vimentin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Vim locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Vim. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Vim function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Vim-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.