ULK1 Double Nickase Plasmid (h): sc-400516-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ULK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ULK1 Double Nickase Plasmid (h) and ULK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ULK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ULK1 Antibody (F-4): sc-390904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ULK1 Double Nickase Plasmid (h) | sc-400516-NIC | 20 µg | $410.00 | |||
ULK1 Double Nickase Plasmid (h2) | sc-400516-NIC-2 | 20 µg | $410.00 |
ULK1 (UNC-51 like autophagy activating kinase 1) is a serine/threonine kinase that initiates autophagy by integrating nutrient and energy signals from mTORC1 and AMPK. As the catalytic core of the ULK1 complex, it phosphorylates downstream targets to promote phagophore formation and regulate mitophagy, proteostasis, and cellular adaptation to metabolic stress. ULK1-dependent autophagy intersects with innate immune signaling, cell survival pathways, and organelle quality control, processes frequently altered in cancer, neurodegeneration, and inflammatory disease contexts. Dysregulated ULK1 activity has been associated with altered stress tolerance and metabolic rewiring, making it a widely used node for pathway dissection in human cell models.
ULK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ULK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ULK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ULK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ULK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.