UBXD1 Double Nickase Plasmid (h): sc-410159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBXD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBXD1 Double Nickase Plasmid (h) and UBXD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBXN6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBXD1 Double Nickase Plasmid (h) | sc-410159-NIC | 20 µg | $410.00 | |||
UBXD1 Double Nickase Plasmid (h2) | sc-410159-NIC-2 | 20 µg | $410.00 |
Human UBXN6 encodes UBXD1, a UBX domain–containing adaptor that links polyubiquitinated substrates to the AAA+ ATPase VCP/p97, coordinating ubiquitin-dependent protein quality control. UBXD1 participates in processes such as ER-associated degradation, endolysosomal trafficking, and autophagy-lysosome pathways, helping regulate turnover of misfolded or damaged proteins and membrane-associated complexes. Through its coupling to VCP/p97-driven extraction and remodeling events, UBXD1 influences cellular proteostasis and stress responses that are frequently perturbed in neurodegenerative and other protein-aggregation–associated conditions. Perturbation of UBXD1-dependent pathways is therefore relevant for mechanistic studies of ubiquitin signaling, membrane protein homeostasis, and downstream inflammatory or stress-linked signaling networks.
UBXD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBXN6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBXN6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBXN6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBXN6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.