Tris Buffered Saline with Tween 20: powder for 5 L of 10X

用法 :

Reconstitute powder in ddH₂0 and empty contents of Tween-20 vial (rinse vial thoroughly) into liquid, bringing final volume to 100 ml (10X). Filter solution through a 0.22 μm filter into a sterile flask. Makes 1 L of 1X TBST buffer.

Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. Final (1X) pH 7.4 ±0.2 (may need slight pH adjustment).

pH :

When diluted to a 1X concentration, the pH will be ~7.4.

保存方法 :

Store at room temperature