TBK1 Double Nickase Plasmid (m): sc-425191-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TBK1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TBK1 Double Nickase Plasmid (m) and TBK1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tbk1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TBK1 Antibody (A-6): sc-398366
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBK1 Double Nickase Plasmid (m) | sc-425191-NIC | 20 µg | $410.00 | |||
TBK1 Double Nickase Plasmid (m2) | sc-425191-NIC-2 | 20 µg | $410.00 |
Mouse Tbk1 encodes TANK-binding kinase 1 (TBK1), a serine/threonine kinase that integrates innate immune and stress signals by phosphorylating IRF3/IRF7 and modulating NF-κB–linked transcriptional programs. TBK1 operates downstream of pattern-recognition receptor pathways including cGAS–STING and RIG-I/MAVS to drive type I interferon responses and antiviral defense, and it also interfaces with selective autophagy and mitophagy through adaptor proteins such as OPTN and SQSTM1. In addition to host defense, TBK1 signaling influences metabolic homeostasis and inflammatory signaling networks that are frequently studied in neuroinflammation and neurodegeneration models. Dysregulated TBK1 activity or dosage has been linked to immune-related and neurodegenerative phenotypes, making Tbk1 a key node for mechanistic studies in mouse cells.
TBK1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tbk1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tbk1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tbk1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tbk1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.