TAAR5 Double Nickase Plasmid (h): sc-409205-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAAR5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TAAR5 Double Nickase Plasmid (h) and TAAR5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TAAR5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAAR5 Double Nickase Plasmid (h) | sc-409205-NIC | 20 µg | $410.00 | |||
TAAR5 Double Nickase Plasmid (h2) | sc-409205-NIC-2 | 20 µg | $410.00 |
TAAR5 (trace amine-associated receptor 5) encodes a rhodopsin-like G protein–coupled receptor that detects biogenic trace amines and related odorant ligands, contributing to chemosensory signaling. Upon activation, TAAR5 is typically linked to heterotrimeric G protein pathways that modulate second-messenger signaling such as cAMP and downstream transcriptional responses, integrating cues from extracellular metabolites into cellular physiology. Although best characterized in olfactory neurons, TAAR5 expression has also been reported in select peripheral tissues, supporting broader roles in neuromodulatory and metabolic signaling networks. Altered TAAR-family signaling has been investigated in the context of neuropsychiatric phenotypes and sensory processing differences, making TAAR5 a useful target for mechanistic studies of GPCR function and ligand-driven signaling.
TAAR5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TAAR5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAAR5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAAR5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAAR5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.