Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sulfiredoxin Double Nickase Plasmid (h): sc-403023-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sulfiredoxin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sulfiredoxin Double Nickase Plasmid (h) and Sulfiredoxin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SRXN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sulfiredoxin Antibody (H-10): sc-514940
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sulfiredoxin Double Nickase Plasmid (h) | sc-403023-NIC | 20 µg | $410.00 | |||
Sulfiredoxin Double Nickase Plasmid (h2) | sc-403023-NIC-2 | 20 µg | $410.00 |
SRXN1 encodes sulfiredoxin, an ATP-dependent reductase that restores overoxidized 2-Cys peroxiredoxins from the sulfinic acid state, thereby reactivating peroxiredoxin-mediated peroxide detoxification. Through this repair cycle, sulfiredoxin helps maintain cellular redox homeostasis and supports signaling processes influenced by reactive oxygen species, including stress adaptation programs that intersect with NRF2-regulated antioxidant pathways. Altered SRXN1 expression has been linked to oxidative stress phenotypes and has been reported across contexts such as tumor biology, inflammation, and neurodegeneration, where peroxiredoxin function and redox buffering can shape cell fate decisions. As a result, SRXN1 is widely studied for its role in regulating peroxide metabolism, protein oxidation, and redox-sensitive signaling networks.
Sulfiredoxin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SRXN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SRXN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SRXN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SRXN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.