StARD9 Double Nickase Plasmid (h): sc-407328-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- StARD9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- StARD9 Double Nickase Plasmid (h) and StARD9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STARD9. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
StARD9 Double Nickase Plasmid (h) | sc-407328-NIC | 20 µg | $410.00 | |||
StARD9 Double Nickase Plasmid (h2) | sc-407328-NIC-2 | 20 µg | $410.00 |
STARD9 encodes a mitotic kinesin-associated protein implicated in centrosome and spindle apparatus function, supporting microtubule organization and accurate chromosome segregation during cell division. StARD9 activity links to cell-cycle progression and mitotic checkpoint control, processes that are frequently dysregulated in proliferative disorders. Altered STARD9 expression or function has been associated with genomic instability phenotypes, making it a relevant target for studying mechanisms that couple spindle dynamics to aneuploidy. In human cell models, perturbation of StARD9 provides a strategy to interrogate mitosis-linked signaling networks and stress responses that influence cell viability.
StARD9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STARD9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STARD9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STARD9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STARD9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.