SPT5 Antibody (E-8): sc-137231

SPT5 Antibody (E-8) is a mouse monoclonal IgG1 antibody that detects SPT5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPT5, also known as DSIF p160, plays a crucial role in the regulation of transcriptional elongation, particularly in the context of RNA polymerase II activity. SPT5 is primarily located in the nucleus, where SPT5 forms a complex with SPT4 to create the DRB-sensitivity-inducing factor (DSIF) complex, which is essential for modulating the transcription process. The interaction between SPT4 and SPT5 is vital for the recruitment of RNA polymerase II, thereby influencing the efficiency of gene expression. Notably, SPT5 undergoes post-translational modifications, including phosphorylation, which significantly alters mobility on SDS-PAGE and enhances regulatory functions. The C-terminal CTR1 domain of SPT5 is specifically phosphorylated by the P-TEFb complex, a modification that is critical for transcriptional elongation. Anti-SPT5 antibody (E-8) enables researchers to gain insights into the dynamics of transcription regulation and the molecular mechanisms underlying gene expression in various biological contexts.

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SPT5 Antibody (E-8) References:

1. Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster.  |  Kaplan, CD., et al. 2000. Genes Dev. 14: 2623-34. PMID: 11040216
2. Sub1 associates with Spt5 and influences RNA polymerase II transcription elongation rate.  |  García, A., et al. 2012. Mol Biol Cell. 23: 4297-312. PMID: 22973055
3. The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis.  |  Dürr, J., et al. 2014. Nucleic Acids Res. 42: 4332-47. PMID: 24497194
4. Structures and Functions of the Multiple KOW Domains of Transcription Elongation Factor Spt5.  |  Meyer, PA., et al. 2015. Mol Cell Biol. 35: 3354-69. PMID: 26217010
5. Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification.  |  Mbogning, J., et al. 2015. Nucleic Acids Res. 43: 9766-75. PMID: 26275777
6. Regulation of RNA polymerase II processivity by Spt5 is restricted to a narrow window during elongation.  |  Fitz, J., et al. 2018. EMBO J. 37: PMID: 29514850
7. The transcription factor Spt4-Spt5 complex regulates the expression of ATG8 and ATG41.  |  Wen, X., et al. 2020. Autophagy. 16: 1172-1185. PMID: 31462158
8. ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.  |  Park, K., et al. 2022. Nucleic Acids Res. 50: 3835-3851. PMID: 35325203
9. The pausing zone and control of RNA polymerase II elongation by Spt5: Implications for the pause-release model.  |  Fong, N., et al. 2022. Mol Cell. 82: 3632-3645.e4. PMID: 36206739
10. SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation.  |  Cui, Y., et al. 2012. Am J Mol Biol. 2: 11-20. PMID: 36910576

The preferred SPT5 antibody is SPT5 Antibody (D-3): sc-133217.