SOCS-5 CRISPR/Cas9 KO Plasmid (h): sc-402585
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SOCS-5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SOCS-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SOCS-5 Antibody (31-L): sc-100858
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SOCS-5 CRISPR/Cas9 KO Plasmid (h) | sc-402585 | 20 µg | $397.00 |
Overview
SOCS5 encodes suppressor of cytokine signaling 5 (SOCS-5), a member of the SOCS family that attenuates cytokine and growth factor receptor signaling via SH2-mediated target recognition and SOCS box–dependent recruitment of ubiquitin ligase complexes. SOCS-5 is implicated in negative regulation of JAK/STAT signaling and can modulate receptor-associated pathways linked to immune cell activation, inflammatory responses, and differentiation programs. Through controlling signal amplitude and duration, SOCS-5 contributes to feedback regulation of cytokine-driven transcriptional outputs and cross-talk with MAPK-associated processes. Dysregulated SOCS5 expression or function has been associated with aberrant immune signaling and has been investigated in contexts of cancer-related signaling rewiring and inflammatory disease mechanisms.
SOCS-5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOCS5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOCS5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOCS5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SOCS-5 protein expression.
This CRISPR knockout system enables efficient generation of SOCS5-deficient cell models for investigation of SOCS-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOCS5 exon(s) critical for SOCS-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOCS5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SOCS-5 CRISPR/Cas9 KO Plasmid (h) and SOCS-5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOCS5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SOCS-5 HDR Plasmid (h) and SOCS-5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOCS5 homology arms to support homology-directed repair at defined SOCS5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.