SMN Antibody (F-5): sc-365909

SMN Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects SMN in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMN, or survival of motor neuron, is a crucial protein involved in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing in the nucleus. The proper functioning of SMN is vital for maintaining the health of motor neurons, as SMN deficiency leads to spinal muscular atrophy, a severe neurodegenerative disorder characterized by the loss of these neurons. SMN exists in multiple isoforms due to alternative splicing and forms a complex with several other proteins, including Gemin2, Gemin3, and various spliceosomal snRNP proteins, facilitating the assembly and recycling of splicing factors. SMN localization in both the cytoplasm and nucleus is significant; in the cytoplasm, SMN interacts with spliceosomal Sm proteins to promote their assembly onto U snRNAs, while in the nucleus, SMN is concentrated in subnuclear bodies known as gems, where SMN plays a role in the recycling of splicing factors. SMN expression across various tissues, including the brain, kidney, liver, spinal cord, and muscle, underscores SMN importance in cellular function and highlights the potential impact of SMN dysfunction in disease states. Anti-SMN antibody (F-5) is an invaluable tool for researchers studying the molecular mechanisms underlying spinal muscular atrophy and related disorders.

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SMN Antibody (F-5) References:

1. Phosphoregulation of the human SMN complex.  |  Husedzinovic, A., et al. 2014. Eur J Cell Biol. 93: 106-17. PMID: 24602413
2. SMN affects membrane remodelling and anchoring of the protein synthesis machinery.  |  Gabanella, F., et al. 2016. J Cell Sci. 129: 804-16. PMID: 26743087
3. Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.  |  Iyer, CC., et al. 2018. Hum Mol Genet. 27: 3404-3416. PMID: 29982416
4. Conditional deletion of SMN in cell culture identifies functional SMN alleles.  |  Blatnik, AJ., et al. 2020. Hum Mol Genet. 29: 3477-3492. PMID: 33075805
5. Heat increases full-length SMN splicing: promise for splice-augmenting therapies for SMA.  |  Dominguez, CE., et al. 2022. Hum Genet. 141: 239-256. PMID: 35088120
6. The phospho-landscape of the survival of motoneuron protein (SMN) protein: relevance for spinal muscular atrophy (SMA).  |  Detering, NT., et al. 2022. Cell Mol Life Sci. 79: 497. PMID: 36006469
7. SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members.  |  Binda, O., et al. 2023. Life Sci Alliance. 6: PMID: 36375840
8. NSC Physiological Features in Spinal Muscular Atrophy: SMN Deficiency Effects on Neurogenesis.  |  Adami, R. and Bottai, D. 2022. Int J Mol Sci. 23: PMID: 36499528
9. SMN Deficiency Destabilizes ABCA1 Expression in Human Fibroblasts: Novel Insights in Pathophysiology of Spinal Muscular Atrophy.  |  Gabanella, F., et al. 2023. Int J Mol Sci. 24: PMID: 36769246
10. A spinal muscular atrophy modifier implicates the SMN protein in SNARE complex assembly at neuromuscular synapses.  |  Kim, JK., et al. 2023. Neuron. 111: 1423-1439.e4. PMID: 36863345

The preferred SMN antibody is SMN Antibody (2B1): sc-32313.