SLC35A3 Double Nickase Plasmid (hamster): sc-437326-NIC
- Target species:
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC35A3 Double Nickase Plasmid (hamster) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC35A3 Double Nickase Plasmid (hamster) and SLC35A3 Double Nickase Plasmid (hamster2) encode distinct paired gRNA designs targeting . One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC35A3 Double Nickase Plasmid (hamster) | sc-437326-NIC | 20 µg | $410.00 | |||
SLC35A3 Double Nickase Plasmid (hamster2) | sc-437326-NIC-2 | 20 µg | $410.00 |
SLC35A3 encodes a Golgi/ER-associated nucleotide-sugar transporter that mediates the import of UDP-N-acetylglucosamine into the secretory pathway, supporting protein and lipid glycosylation. By regulating substrate availability for glycosyltransferases, SLC35A3 influences N-glycosylation, O-glycosylation, and proteoglycan biosynthesis, thereby shaping trafficking, stability, and signaling of membrane and secreted proteins. Perturbation of this transporter can alter glycan-dependent processes such as cell adhesion, receptor maturation, and immune recognition, making it relevant to studies of congenital disorders of glycosylation and glycosylation-linked metabolic or neurodevelopmental phenotypes.
SLC35A3 Double Nickase Plasmid (hamster) consists of a matched pair of plasmids engineered for high-specificity editing of the locus in cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within . When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of -disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.