Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SH-PTP1 Double Nickase Plasmid (h): sc-400369-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SH-PTP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SH-PTP1 Double Nickase Plasmid (h) and SH-PTP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTPN6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SH-PTP1 Antibody (D-11): sc-7289
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH-PTP1 Double Nickase Plasmid (h) | sc-400369-NIC | 20 µg | $410.00 | |||
SH-PTP1 Double Nickase Plasmid (h2) | sc-400369-NIC-2 | 20 µg | $410.00 |
PTPN6 encodes the cytosolic protein tyrosine phosphatase SH-PTP1 (SHP-1), a key negative regulator of signaling downstream of immune receptors and cytokine receptors. By dephosphorylating activated kinases and receptor complexes, SHP-1 dampens JAK/STAT, MAPK/ERK, and PI3K/AKT pathway outputs and helps maintain thresholds for activation, differentiation, and inflammatory responses. In human systems, altered PTPN6 activity has been linked to dysregulated immune signaling, chronic inflammation, and immune-oncology contexts, making it a useful node for studying feedback control in hematopoietic and immune cell models. Functional perturbation of SHP-1 also informs research on phosphatase-kinase network balance and receptor-proximal signaling dynamics.
SH-PTP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTPN6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTPN6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTPN6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTPN6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.