RecQL5 Double Nickase Plasmid (h): sc-406407-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RecQL5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RecQL5 Double Nickase Plasmid (h) and RecQL5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RECQL5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RecQL5 Antibody (C-9): sc-515050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RecQL5 Double Nickase Plasmid (h) | sc-406407-NIC | 20 µg | $410.00 | |||
RecQL5 Double Nickase Plasmid (h2) | sc-406407-NIC-2 | 20 µg | $410.00 |
RECQL5 encodes RecQ helicase family member 5, a DNA helicase that helps preserve genome stability by regulating DNA replication dynamics and coordinating repair of DNA lesions. RecQL5 interacts with RNA polymerase II and contributes to control of transcription-associated recombination, limiting aberrant crossover events and replication–transcription conflicts. Through roles in homologous recombination regulation, replication fork maintenance, and responses to DNA damage signaling, RECQL5 is frequently studied in pathways that constrain mutagenesis and chromosomal rearrangements. Altered RECQL5 function has been associated with increased genomic instability phenotypes that are relevant to mechanistic research on cancer susceptibility and genome maintenance disorders.
RecQL5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RECQL5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RECQL5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RECQL5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RECQL5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.