Rak Double Nickase Plasmid (h): sc-405590-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rak Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rak Double Nickase Plasmid (h) and Rak Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FRK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rak Antibody (H-12): sc-166478
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rak Double Nickase Plasmid (h) | sc-405590-NIC | 20 µg | $410.00 | |||
Rak Double Nickase Plasmid (h2) | sc-405590-NIC-2 | 20 µg | $410.00 |
Human FRK encodes the non-receptor tyrosine kinase Rak, a Src family–related enzyme enriched in epithelial contexts that modulates signaling from growth factor receptors and cell–cell adhesion complexes. Rak participates in phosphorylation-dependent control of cytoskeletal dynamics, cell-cycle progression, and differentiation programs through pathways that intersect with EGFR/ErbB signaling, focal adhesion signaling, and junctional regulation. Altered FRK activity or expression has been linked to dysregulated proliferation and epithelial homeostasis, making it relevant to studies of tumor biology, invasion, and lineage-specific signaling. As a kinase with context-dependent effects on growth and motility, Rak is frequently investigated to map phosphorylation networks and feedback control in stress and receptor-driven signaling.
Rak Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FRK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FRK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FRK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FRK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.