POGZ CRISPR Activation Plasmid (h): sc-410566-ACT

POGZ (pogo transposable element-derived protein with zinc finger) is a nuclear chromatin-associated factor that regulates transcriptional programs by coordinating DNA-binding and protein–protein interactions at gene regulatory regions. It contributes to chromatin organization and genome stability by influencing mitotic progression and sister chromatid cohesion through interactions with heterochromatin-linked machinery, impacting cell cycle control and developmental gene expression networks. Dysregulation of POGZ-dependent chromatin regulation has been associated with neurodevelopmental phenotypes and altered neuronal differentiation, making it relevant for studies of transcriptional control in brain development. In human cells, POGZ is frequently examined in the context of epigenetic regulation, cell fate specification, and pathway-level effects on RNA expression programs.

POGZ CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous POGZ expression without altering the underlying DNA sequence.

POGZ CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the POGZ locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.

Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the POGZ transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous POGZ expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native POGZ locus and enabling the study of POGZ-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of POGZ pathway restoration in tumor cells with silenced or reduced POGZ expression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.