• To prepare PNPP substrate solution, dissolve 10 mg of room-temperature PNPP, Disodium Salt (sc-3720) into 10 ml of pNPP Substrate Buffer (sc-296099). Mix well and protect from light. Diluted product may be used for up to 10 hours following mixing. Discard if solution becomes yellow or turbid.
• Following incubation of antibody samples and the addition of alkaline phosphatase labeled probes, wash plates thoroughly avoiding the use of phosphate buffers. Add 100 µl of 1X PNPP substrate solution to each well and incubate 15-60 minutes. Incubation times will vary depending on the enzyme present.
• For endpoint analysis, the reaction may be stopped with 3N NaOH and read at 405 nm.
外観 :
Crystalline powder
身体の状態 :
Solid
溶解度 :
Soluble in water(freely soluble). Insoluble in ethanol, and acetone.