PLD6 Double Nickase Plasmid (h): sc-410373-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLD6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PLD6 Double Nickase Plasmid (h) and PLD6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLD6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLD6 Double Nickase Plasmid (h) | sc-410373-NIC | 20 µg | $410.00 |
Human PLD6 encodes a mitochondrial outer membrane phospholipase D family endonuclease also known as MitoPLD that generates phosphatidic acid from cardiolipin, linking lipid metabolism to mitochondrial dynamics. PLD6 activity contributes to mitochondrial fusion and architecture, and it participates in small RNA biogenesis pathways in germ cells through roles in piRNA processing and transposon silencing. By influencing mitochondrial membrane remodeling and genome defense mechanisms, PLD6 is relevant to studies of germline integrity, meiosis, and cellular stress responses. Dysregulation of these processes has been associated with reproductive phenotypes and mitochondrial dysfunction–related cellular defects, supporting PLD6 as a target for mechanistic investigation.
PLD6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLD6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLD6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLD6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLD6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.