PiT1 Double Nickase Plasmid (m): sc-422988-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PiT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PiT1 Double Nickase Plasmid (m) and PiT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc20a1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PiT1 Double Nickase Plasmid (m) | sc-422988-NIC | 20 µg | $410.00 |
Mouse Slc20a1 encodes the type III sodium-dependent phosphate transporter PiT1, a broadly expressed membrane protein that mediates inorganic phosphate uptake to support nucleotide synthesis, phospholipid metabolism, and cellular bioenergetics. PiT1 integrates phosphate availability with cell-cycle progression and survival signaling, and has been linked to proliferation control and stress responses in multiple cell types. In addition to phosphate homeostasis, PiT1 contributes to mineralization-related processes and phosphate-driven signaling networks that intersect with metabolic and developmental pathways. Dysregulation of phosphate transport and PiT1-associated pathways is relevant to studies of calcification biology, metabolic remodeling, and proliferative phenotypes in disease-relevant model systems.
PiT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc20a1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc20a1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc20a1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc20a1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.