PHF8 CRISPR/Cas9 KO Plasmid (h): sc-407306
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHF8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF8 CRISPR/Cas9 KO Plasmid (h) | sc-407306 | 20 µg | $397.00 |
Overview
PHF8 encodes a JmjC domain–containing histone demethylase that recognizes H3K4me3 via its PHD finger and removes repressive lysine methylation marks such as H3K9me2/1 and H4K20me1 to modulate chromatin accessibility. Through epigenetic control of transcription, PHF8 contributes to cell-cycle progression, DNA damage responses, and lineage-specific differentiation programs. PHF8 function intersects with chromatin remodeling and transcriptional regulation pathways that shape gene expression networks during development. Dysregulation or mutation of PHF8 has been associated with neurodevelopmental phenotypes and altered transcriptional states observed in cancer-related epigenetic reprogramming.
PHF8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHF8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHF8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHF8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHF8 protein expression.
This CRISPR knockout system enables efficient generation of PHF8-deficient cell models for investigation of PHF8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHF8 exon(s) critical for PHF8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHF8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHF8 CRISPR/Cas9 KO Plasmid (h) and PHF8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHF8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHF8 HDR Plasmid (h) and PHF8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHF8 homology arms to support homology-directed repair at defined PHF8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.