PGS1 Double Nickase Plasmid (h): sc-411287-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PGS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PGS1 Double Nickase Plasmid (h) and PGS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PGS1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PGS1 Double Nickase Plasmid (h) | sc-411287-NIC | 20 µg | $410.00 |
PGS1 encodes phosphatidylglycerophosphate synthase 1, a mitochondrial inner membrane enzyme that catalyzes the committed step in phosphatidylglycerol production, a precursor required for cardiolipin biosynthesis. Through its role in the cardiolipin remodeling and mitochondrial phospholipid homeostasis network, PGS1 influences oxidative phosphorylation efficiency, cristae architecture, and stress-responsive mitochondrial quality control. Perturbation of PGS1 activity can shift membrane lipid composition and impact mitochondrial dynamics and bioenergetic signaling pathways. Accordingly, PGS1 is studied in the context of mitochondrial dysfunction phenotypes and lipid metabolism–linked disease mechanisms where cardiolipin availability is a key determinant of organelle performance.
PGS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PGS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PGS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PGS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PGS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.