PCIF1 Double Nickase Plasmid (h): sc-407384-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCIF1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PCIF1 Double Nickase Plasmid (h) and PCIF1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PCIF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PCIF1 Antibody (A-9): sc-374406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCIF1 Double Nickase Plasmid (h) | sc-407384-NIC | 20 µg | $410.00 | |||
PCIF1 Double Nickase Plasmid (h2) | sc-407384-NIC-2 | 20 µg | $410.00 |
PCIF1 (phosphorylated CTD interacting factor 1) is an mRNA cap-associated methyltransferase that catalyzes N6-methylation of 2′-O-methyladenosine at the first transcribed nucleotide (m6Am) adjacent to the m7G cap, shaping cap-proximal RNA modification landscapes. Through this activity, PCIF1 influences mRNA stability, translation efficiency, and transcript-specific gene expression programs that intersect with RNA processing and epitranscriptomic regulation. PCIF1-dependent m6Am has been linked to modulation of cellular stress responses and developmental and proliferative signaling outputs via post-transcriptional control. Dysregulation of cap-proximal methylation and PCIF1 expression has been investigated in contexts including tumor biology and immune-related gene expression, supporting its relevance for mechanistic studies of RNA modification–driven phenotypes.
PCIF1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PCIF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PCIF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PCIF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PCIF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.