Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PAR4 Double Nickase Plasmid (h): sc-401142-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAR4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PAR4 Double Nickase Plasmid (h) and PAR4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PAWR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PAR4 Antibody (A-10): sc-1666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAR4 Double Nickase Plasmid (h) | sc-401142-NIC | 20 µg | $410.00 | |||
PAR4 Double Nickase Plasmid (h2) | sc-401142-NIC-2 | 20 µg | $410.00 |
PAWR encodes the pro-apoptotic protein PAR4 (prostate apoptosis response-4), a tumor suppressor–associated factor that modulates intrinsic and extrinsic apoptosis pathways and antagonizes pro-survival signaling. PAR4 influences transcriptional and stress-response programs through interactions with atypical PKC isoforms, NF-κB regulatory nodes, and apoptotic machinery, linking it to control of cell fate decisions under genotoxic and inflammatory stress. Altered PAWR/PAR4 expression has been reported across multiple human cancers and is associated with changes in apoptosis sensitivity, cellular differentiation, and metastatic behavior. In addition to oncology contexts, PAR4 has been implicated in neurodegeneration and other disorders where dysregulated cell death and stress signaling contribute to pathology.
PAR4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PAWR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PAWR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PAWR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PAWR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.