p-PKC beta 2/delta Antibody (E-7): sc-365463

p-PKC βII/δ Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects p-PKC βII/δ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-PKC βII/δ (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The protein kinase C (PKC) family, to which p-PKC βII/δ belongs, is crucial for regulating numerous cellular processes, including cell growth, differentiation, and apoptosis. Specifically, p-PKC βII/δ is involved in the phosphorylation of serine residues, which is essential for activation and function. This phosphorylation is a key post-translational modification that influences p-PKC βII/δ activity and interactions with other signaling molecules. p-PKC βII/δ modulates various signaling pathways, underscoring importance in cellular responses to external stimuli and making p-PKC βII/δ a significant target for research in cancer, cardiovascular diseases, and neurodegenerative disorders. Understanding p-PKC βII/δ phosphorylation status can provide insights into disease progression mechanisms and potential therapeutic interventions.

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p-PKC beta 2/delta Antibody (E-7) References:

1. Protein kinase C delta (PKC delta): activation mechanisms and functions.  |  Kikkawa, U., et al. 2002. J Biochem. 132: 831-9. PMID: 12473183
2. PKC-delta binds to E-cadherin and mediates EGF-induced cell scattering.  |  Singh, R., et al. 2009. Exp Cell Res. 315: 2899-913. PMID: 19591825
3. Synthetic Lethality Induced by Loss of PKC δ and Mutated Ras.  |  Zhu, T., et al. 2010. Genes Cancer. 1: 142-51. PMID: 21031151
4. PKC-δ/PKC-α activity balance regulates the lethal effects of cisplatin.  |  Muscella, A., et al. 2015. Biochem Pharmacol. 98: 29-40. PMID: 26300055
5. Dexmedetomidine-Induced Contraction in the Isolated Endothelium-Denuded Rat Aorta Involves PKC-δ-mediated JNK Phosphorylation.  |  Yu, J., et al. 2015. Int J Med Sci. 12: 727-36. PMID: 26392810
6. Activation of RhoA, Smad2, c-Src, PKC-βII/δ and JNK in atopic dermatitis.  |  Lu, J., et al. 2018. Australas J Dermatol. 59: e258-e261. PMID: 29498756
7. PKC-βII is downregulated in the premature ovarian failure SD rat model.  |  Ubba, V., et al. 2023. Reprod Biol. 23: 100754. PMID: 36934663
8. CDDO regulates central and peripheral sensitization to attenuate post-herpetic neuralgia by targeting TRPV1/PKC-δ/p-Akt signals.  |  Lu, CC., et al. 2024. J Cell Mol Med. 28: e18131. PMID: 38426931
9. Inhibition of PKC-δ retards kidney fibrosis via inhibiting cGAS-STING signaling pathway in mice.  |  Wang, D., et al. 2024. Cell Death Discov. 10: 314. PMID: 38972937
10. Association of PKC delta and active Src in PMA-treated MCF-7 human breast cancer cells.  |  Shanmugam, M., et al. 1998. Oncogene. 16: 1649-54. PMID: 9582012

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKC beta 2/delta Antibody (E-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKC beta 2/delta Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKC beta 2/delta Antibody (E-7) Specifications

Alternate Product Name: PKC beta 2/delta

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Ser 660

Epitope Target: PKC βII

Epitope Species: human

Product Reactivity/Detection: Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly Ser 662 phosphorylated PKC δ of mouse and rat origin, and correspondingly Ser 664 phosphorylated PKC δ of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for p-PKC beta 2/delta Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PKC antibody is PKC Antibody (A-9): sc-17804.