Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OTUB1 Double Nickase Plasmid (h): sc-407665-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OTUB1 Double Nickase Plasmid (h) and OTUB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OTUB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OTUB1 Antibody (J-61): sc-130458
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUB1 Double Nickase Plasmid (h) | sc-407665-NIC | 20 µg | $410.00 | |||
OTUB1 Double Nickase Plasmid (h2) | sc-407665-NIC-2 | 20 µg | $410.00 |
OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1) is a cysteine protease deubiquitinase that preferentially cleaves K48-linked ubiquitin chains and can also inhibit E2 ubiquitin-conjugating enzymes, thereby shaping ubiquitin signaling outputs. Through these activities, OTUB1 regulates protein stability and signaling dynamics in pathways controlling DNA damage responses, cell-cycle progression, and stress signaling, including modulation of p53 pathway components and repair-associated ubiquitination events. OTUB1-dependent tuning of ubiquitin-dependent proteostasis influences immune and inflammatory signaling nodes and can affect the turnover of key regulatory proteins. Dysregulated OTUB1 expression or activity has been reported across multiple cancer contexts and other disorders where ubiquitin homeostasis and genome maintenance are perturbed, supporting its utility as a mechanistic target in pathway-focused research.
OTUB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OTUB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OTUB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OTUB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OTUB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.