Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Oma1 CRISPR/Cas9 KO Plasmid (h): sc-402953
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Oma1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Oma1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Oma1 Antibody (H-11): sc-515788
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oma1 CRISPR/Cas9 KO Plasmid (h) | sc-402953 | 20 µg | $397.00 |
Overview
OMA1 encodes a stress-activated metalloprotease embedded in the inner mitochondrial membrane that contributes to mitochondrial quality control by proteolytically processing substrates such as OPA1 to remodel cristae architecture and regulate mitochondrial fusion–fission dynamics. Oma1 activity is induced by mitochondrial depolarization and proteotoxic stress, integrating signals that influence oxidative phosphorylation efficiency, reactive oxygen species handling, and mitophagy-linked surveillance. Through its impact on organelle morphology and bioenergetics, OMA1 is studied in pathways governing apoptosis susceptibility and metabolic adaptation. Dysregulated OMA1–OPA1 axis function has been associated with mitochondrial dysfunction phenotypes relevant to neurodegeneration, cardiometabolic stress, and other disorders where impaired mitochondrial dynamics are implicated.
Oma1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OMA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OMA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OMA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Oma1 protein expression.
This CRISPR knockout system enables efficient generation of OMA1-deficient cell models for investigation of Oma1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OMA1 exon(s) critical for Oma1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OMA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Oma1 CRISPR/Cas9 KO Plasmid (h) and Oma1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OMA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Oma1 HDR Plasmid (h) and Oma1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OMA1 homology arms to support homology-directed repair at defined OMA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.